Anomalous values of Mycoplasma genomes sizes determined by pulse-field gel electrophoresis
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چکیده
منابع مشابه
Pulse-Field Gel Electrophoresis (PFGE) of Salmonella Serovar Infantis Isolates From Poultry
Background: Salmonella is one of the most important zoonotic agents known to infect humans and a wide range of animals, including poultry. Salmonella Infantis has been one of the 15 most frequently isolated serovars throughout the world. Despite its clinical importance, little is known about the molecular characteristics of S. Infantis strains from Iran. OBJECTIVES: The purpose of this study wa...
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The technique of pulse-field gel electrophoresis (PFGE) makes use of restriction enzymes that cleave DNA at specific restriction sites, generating DNA fragments of different sizes. These fragments can be separated according to size by agarose-gel electrophoresis and visualized using DNA stains (e.g. ethidium bromide), giving a characteristic banding pattern. Given the high specificity of restri...
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The chromosomal DNAs of nine strains of seven Bacteroides species including B. fragilis, the type species of the genus Bacteroides, were digested with rare-cutting restriction enzymes I-Ceu I, Not I, and Asc I and analysed by pulsed-field gel electrophoresis. The genome sizes of B. fragilis, B. distasonis, B. eggerthii, B. ovatus, B. thetaiotaomicron, B. uniformis, and B. vulgatus were determin...
متن کاملPulsed-field gel electrophoresis.
DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
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Pulse field gel electrophoresis (PFGE) was utilised to compare the genomes of 16 Streptococcus thermophilus cultures from yoghurt, cheese, laban and dahi after digestion with the restriction endonucleases, SfiI, SmaI and BssHII. PFGE profiles could be used for strain identification and were also useful in predicting relatedness of certain strains. Genetic variations between specific morphotypes...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1989
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/17.3.1268